9P - Distribution of T cells in non-small cell lung cancer tissue according to COPD and smoking status

Date 17 April 2015
Event ELCC 2015
Session Poster lunch
Topics Non-Small-Cell Lung Cancer, Metastatic
Pathology/Molecular Biology
Translational Research
Presenter Jurgita Jackute
Citation Annals of Oncology (2015) 26 (suppl_1): 1-5. 10.1093/annonc/mdv043
Authors J. Jackute1, M. Zemaitis1, D. Pranys2, B. Sitkauskiene1, S. Miliauskas1, R. Sakalauskas1
  • 1Pulmonology And Immunology, Medical Academy, Lithuanian University of Health Sciences, LT-50009 - Kaunas/LT
  • 2Pathology, Hospital of Lithuanian University of Health Sciences, LT-50009 - Kaunas/LT

Abstract

Aim/Background

The immune system and smoking are believed to play important roles in the pathogenesis of non-small cell lung cancer (NSCLC) and chronic obstructive pulmonary disease (COPD). The aim of this study was to clarify the differences of tumor infiltrating T cells (CD4+ and CD8+) according to COPD and cigarette smoking status.

Methods

We studied 50 NSCLC patients (stages I-III) with median age 64.3 years (range 45-77). Immunohistochemical analysis of surgical lung biopsy samples was performed. Quantitative evaluation of CD4+ and CD8+ T cells in tumor islets and stroma were analyzed in the 5 most representative high-power fields (HPFs x 400 magnification) per tissue section using an Olympus BX50 microscope.

Results

More CD8+ T cells in tumor stroma were presented in NSCLC with COPD compared with NSCLC without COPD patients (159 [78-227] vs. 129 [47-230], accordingly; P = 0.04). More CD8+ T cells in tumor islets were found in NSCLC patients without COPD compared with NSCLC patients with COPD (24 [10-135] vs. 21 [6-46], accordingly; P = 0.01). We did not observe any significant differences of CD4+ T cell amounts in tumor stroma, islets and total CD4+ T cells number in NSCLC without COPD and NSCLC with COPD patients groups. Total numbers of tumor infiltrating CD8+ T cells did not differ between these groups, but the tendency that higher numbers of tumor infiltrating CD8+ T cells are in the NSCLC patients with COPD group was observed. We found significantly higher amounts of stromal tumor infiltrating CD4+ T cells (155 [40-326] vs. 86 [49-147], accordingly; P = 0.02) and total tumor infiltrating CD4+ T cells (160.5 [53-348] vs. 110.5 [60-155], accordingly; P = 0.03) in smoking patients group compared with non-smokers NSCLC patients. Also greater numbers of CD8+ T cells in cancer stroma (141.5 [49-230] vs. 103.5 [47-179], accordingly; P = 0.04) and total tumor infiltrating CD8+ T cells (168.5 [67-307] vs. 142.5 [57-205], accordingly; P = 0.03) were found in smoking NSCLC patients compared with non-smoker NSCLC patients. There were no differences of CD4+ and CD8+ T cell distribution in tumor islets in these groups.

Conclusions

Smoking as well as COPD influences differently CD4+ and CD8+ T cell distribution in non-small cell lung cancer tissue.

Clinical trial identification NCT01955343; September 27, 2013

Disclosure

All authors have declared no conflicts of interest.