1212P - Circulating tumor cells in peripheral and pulmonary venous blood predict poor long-term survival in surgically resected non-small cell lung cancer...

Date 27 September 2014
Event ESMO 2014
Session Poster Display session
Topics Non-Small-Cell Lung Cancer, Locally Advanced
Translational Research
Presenter ZhiDong Liu
Citation Annals of Oncology (2014) 25 (suppl_4): iv417-iv425. 10.1093/annonc/mdu348
Authors Z. Liu1, R. Zhang2, Y. Li1, S. Xu1, Y. Han1, C. Su1, Z. Chen3, D. Zhen1
  • 1Thoracic Surgery, Beijing Chest Hospital, Capital Medical University, 101149 - Beijing/CN
  • 2Hematology, Beijing Children’s Hospital, Capital Medical University, Beijing/CN
  • 3Clinical Genomics Unit, Head And Neck Surgery Branch, National Institute on Deafness and Communication Disorders, National Institutes of Health, Bethesda/US



We tested the hypothesis that the circulating tumor cells (CTCs) in preoperative peripheral blood (PPB) and intraoperative pulmonary venous blood (IPVB) could predict poor long-term survival in surgically resected NSCLC patients.


CTCs were separated from the blood using magnetic beads coated with antibody against epithelial-cell adhesion molecule (EpCAM) through magnetic activated cell sorting (MACS). The CTCs were quantified with fluorescence-labeled antibodies against pan-cytokeratin through flow cytometry. CTCs were prospectively quantified in PPB and IPVB in 23 consecutive stage I-IIIA patients with surgically resected NSCLC. Association between CTCs and prognosis of these patients was evaluated after 5-year follow-up.


In the NSCLC patients, outcomes were assessed according to levels of CTCs at surgery, and compared with CTCs detected in benign pulmonary diseases, and healthy volunteers, where the mean and 95% CI of CTCs counts were all <1.0 CTCs/15 mL. In cancer patients, the median of PPB-CTCs was 5/15mL, and the median of IPVB-CTCs was 28/15mL. NSCLC patients were identified as high-risk groups, as >5 CTCs/15mL in PPB and >50 CTCs/15mL in IPVB. Univariate Cox proportional-hazards regression analysis showed that CTCs count in PPB or IPVB was an independent risk factor for tumor-free and overall survivals. The high risk group of patients had a shorter median tumor-free survival (22 months vs. >60.0 months, P<0.0012) and shorter overall survival (27 months vs. >60 months, P < 0.0015).


CTCs count in PPB and IPVB was an independent risk factor for tumor-free and overall survival in surgically resected NSCLC patients.


All authors have declared no conflicts of interest.