1055 - Efficacy of myofibroblasts as an indicator for invasion and nodal metastasis in OSCC

Date 28 September 2012
Event ESMO Congress 2012
Session Publication Only
Topics Head and Neck Cancers
Translational Research
Presenter Amsavardani Tayaar Sivakumar
Authors A.T. Sivakumar1, P. Sowmya2, B.M. Jyothi2, M.V. Muddapur3
  • 1Oral Pathology, S D M College of Dental Sciences & Hospital,, 580009 - Dharwad/IN
  • 2Oral Pathology, SDM College of Dental Sciences, 580009 - Dharwad/IN
  • 3Statistics, Karnataka University, Dharwad/IN

Abstract

Purpose

Tumour cells work in close coordination with stromal elements from its stage of initiation to metastasis. Oral squamous cell carcinoma (OSCC) is one of the top ten cancers in the world with only survival rate of 56%. Myofibroblasts (MF), a cell that is identified transiently in the wounds have also been shown in tumor stromogenesis in a variety of malignancies. MFs been highlighted for their proinvasive role in tumors. These cells remain less explored in OSCC in terms of invasion and nodal metastasis – Prognosticators.

Method

The study was aimed towards understanding the possibility of MF being an indicator of invasion and nodal metastasis. This was achieved through assessing the presence and distribution pattern of MF in OSCC using α-SMA. To improve further our understanding a semiquantative analysis (0= No staining, 1= Focal Positivity, 2 = Multifocal Positivity) was performed and compared with that from normal mucosa (NM, number of cases (n) =10), chronic inflammatory lesions (CIL, n= 22), surgical margins of OSCC patients (SM, n = 24) and pN0, pN+ OSCC samples (n = 56, 28 each). Mann-Whitney test was applied.

Results

No MFs were present in NM and SM. 84% and 32% of OSCC and CIL showed MFs respectively. Heterogenous pattern (Presence/absence in a location, Loose/Syncitial arrangements, and Focal/Multifocal distribution) of MFs was seen in OSCC while they were seen closer to the center of the lesion away from the epithelium in CIL. Lesions reported for longer duration showed MF in CIL. The MFs were not seen in all the invasive fronts. Significant difference in the number of MFs was observed between NM and OSCC (p = 0.00), CIL and SM (p = 0.003), CIL and SCC (p = 0.00), SM and OSCC (p = 0.00).

Conclusion

MF formation and its retention seems to be influenced by duration (reactive lesions), and basement membrane invasion with stressed extracellular matrix (OSCC). Stress-dictated distribution patterns may prove valuable in distinguishing pN0 and pN+ groups. Smaller incisional biopsies may not be useful in predicting both invasion and nodal metastasis either by quantitation or by distribution pattern analysis owing to the heterogeneity that is displayed.

Disclosure

All authors have declared no conflicts of interest.