1663P - Level of estrogens and their metabolites in infected ovarian tissue in women having uterine carcinomas

Date 28 September 2014
Event ESMO 2014
Session Poster Display session
Topics Endometrial Cancer
Pathology/Molecular Biology
Translational Research
Presenter Tatiana Moiseenko
Citation Annals of Oncology (2014) 25 (suppl_4): iv564-iv573. 10.1093/annonc/mdu359
Authors T. Moiseenko1, E. Frantsiyants2, V. Bandovkina2, E. Komarova2, M. Vovkochina1
  • 1Department Of Gynecology, Rostov Research Oncological Institute, 344037 - Rostov-on-Don/RU
  • 2Laboratory Of Pathogenesis Of Malignant Tumors, Rostov Research Oncological Institute, 344037 - Rostov-on-Don/RU

Abstract

Aim

Recently growing evidence has indicated the presence of persistent viruses and microorganisms in malignant tumor cells. Studying of the effect of infectious factors on the changes in endocrine profile in hormone-dependent tissues can contribute to detection of the reasons of sharp rise in the frequency of cancer in women over fifty years and the possibility of prevention and treatment of oncopathologies.

Methods

67 samples of ovarian tissue in patients with uterine carcinomas T1-2N0M0 were studied, mean age 53,4±3,2 years. Levels of estrone (Е1), estradiol (Е2), estriol (Е3), estrogen metabolite ratio 2-ОНЕ and 16-ОНЕ were detected in 10% homogenates by enzyme-linked immunosorbent assay with the help of standard test systems (XEMA Co., Ltd.). Intact ovarian tissue without IgG antibodies against infectious agents served as a control. Polymerase chain reaction method was used for the detection of cytomegalovirus (CMV), herpes simplex virus (HSV) and chlamydia infection in ovarian tissue. Statistical analysis of results was made using Statistica 6.0 programme. Written voluntary informed consent of all patients was received in all cases.

Results

35,8% of 67 samples of ovarian tissue were infected with complex of infections – clamydias+HSV+CMV; 32,8% – HSV only; 11,9% – CMV+HSV; 11,9% – CMV only. Only 7,6% of the samples occurred to be intact. The results of the study show increase of Е1 concentration by 1,6-3,5 times and decrease of Е2 by 7,3-16,4 times in infected ovaries in comparison with uninfected tissues. When calculating the ratio Е12, maximum prevalence of Е1 – by 25-28,8 times higher in comparison with uninfected tissue – was recorded in HSV and HSV+CMV. In all other cases – CMV and clamydias+CMV – Е12 ratio increased by 15-16 times. Estrogen metabolite ratio 2-ОНЕ/16-ОНЕ in infected ovarian tissue was decreased on average by 1,5 times in comparison with intact samples.

Conclusions

Е12 ratio changes towards Е1 prevalence in infected ovarian tissue, and estrogen metabolite ratio decreases towards aggressive 16ОНЕ prevalence.

Disclosure

All authors have declared no conflicts of interest.