P-165 - Specific changes in fecal microbiota may differentiate Pancreatic Cancer patients from healthy individuals

Date 04 July 2015
Event WorldGI 2015
Session Posters
Topics Pancreatic Cancer
Presenter E. Half
Citation Annals of Oncology (2015) 26 (suppl_4): 1-100. 10.1093/annonc/mdv233
Authors E. Half1, N. Keren1, T. Dorfman1, L. Reshef1, I. Lachter1, Y. Kluger1, F. Konikoff2, U. Gphna1
  • 1Rambam Medical Center, Haifa/IL
  • 2Meir Medical Center, Kfar Saba/IL

Abstract

Introduction

Pancreatic cancer (PC) is a leading cause of cancer-related death in western countries. Since most patients have incurable disease at the time of diagnosis, developing a screening method for early detection is mandatory. Due to its metabolic importance, even slight alterations in pancreatic function may affect the composition of gut microbial communities.

Aim; To detect specific changes in the fecal microbiota that differentiate PC patients from healthy individuals.

Methods

Methods: Fecal material was collected from 15 newly diagnosed PC patients (prior to treatment). Stool samples collected previously from healthy subjects scheduled for screening colonoscopy served as controls (n = 15). Gut microbiota was analyzed by 16S rRNA gene amplicon sequencing, using Ion-Torrent technology.

Results

Fecal bacterial composition of PC patients was significantly different from controls (unweighted-UniFrac based ANOSIM R = 0.4361, p < 0.0001) with a 2-fold higher median relative concentration of the phyla Bacteroidetes and Verrucomicrobia and a concomitant decrease in the phyla Firmicutes and Actinobacteria. The genera Sutterella, Veillonella, Bacteroides, Odoribacter and Akkermansia were increased in pancreatic cancer patients relative to controls. Notably, the three latter genera were previously shown to be increased in a mouse model of inflammation-induced colorectal cancer.

Conclusion

Specific fecal microbial differences occur between PC patients and controls. These changes may represent potential biomarkers for this disease.

The changes in microbial communities are currently being studied in a larger cohort of PC patients as well as in earlier stages of the disease such as pancreatic intraepithelial neoplasia and mucinous cyst adenoma.