P-011 - ARHI re-expression inhibits subcutaneous xenograft growth and the lung/liver metastases of human gastric cancer cells (BGC823) in nude mice

Date 04 July 2015
Event WorldGI 2015
Session Posters
Topics Basic Science
Gastric Cancer
Presenter X.B. Hu
Citation Annals of Oncology (2015) 26 (suppl_4): 1-100. 10.1093/annonc/mdv233
Authors X.B. Hu, J.P. Qiu, Y. Xin
  • the First Affiliated Hospital of China Medical University, Shenyang/CN

Abstract

Introduction

Gastric cancer is a common malignancy, and distant metastasis of gastric cancer is the primary mode of treatment failure, and also the main reason of death in patients. Our previous study found that autophagy-related tumor suppressor gene ARHI can inhibit the proliferation invasion and migration of gastric cancer cells in vitro. This study aimed to investigate the effects of ARHI gene on tumor growth and metastasis in nude mice, and to explore the relevant mechanism.

Methods

In this study, ARHI stably transfected BGC823 gastric cancer cell line was used to establish a subcutaneous xenograft model in BALC/C Nu/Nu nude mice. The impact on the growth of subcutaneous xenografts of ARHI gene was evaluated. IHC was used to evaluate the expressions of LC3, P62, Ki67, SP1, VEGF, and p-AKT proteins. Moreover, a hematogenous metastasis model by tail vein injection of BGC823 cancer cells with variable ARHI expression were also established, the impact on lung and liver metastases tumorigenicity of ARHI gene was evaluated

Results

Subcutaneous xenograft experiment showed that re-expression of ARHI gene in gastric cancer cells inhibited the growth of subcutaneous xenograft, down-regulated p62 expression but up-regulated LC3 expression of subcutaneous tumor cells, inhibited the proliferation and angiogenesis, and inhibited PI3K-AKT signaling pathway. The results also showed that re-expression of ARHI gene in BGC823 cancer cells inhibited metastatic tumor development in the lungs and livers of nude mice.

Conclusion

Re-expression of ARHI gene in BGC823 gastric cancer cells inhibited the growth of subcutaneous xenograft and the lung and liver metastases in vivo, the mechanism is probably related to the regulation of autophagy of cancer cells.