37P - Intranasal administration of Ff bacteriophages towards cancer therapy

Date 20 November 2015
Event ESMO Symposium on Immuno-Oncology 2015
Session Welcome reception and general Poster viewing
Topics Cancer Immunology and Immunotherapy
Translational Research
Presenter Eyal Dor-On
Citation Annals of Oncology (2015) 26 (suppl_8): 5-14. 10.1093/annonc/mdv514
Authors E. Dor-On, D. Schneider, B. Solomon
  • Molecular Microbiology And Biotechnology, Tel-Aviv University, 69978 - Tel Aviv/IL



Ff phages are non-lytic bacteria viruses characterized by a filament and flexible structure. Although they have no tropism to mammalian cells, Ff phages can skew tumor associated macrophage polarization towards the anti-tumorigenic M1 phenotype and promote tumor destruction associated with cytotoxic neutrophils infiltration. Interestingly, owing to their unique dimensions, Ff phages were reported to penetrate the central nervous system following intranasal administration. The primary objective of this study was to assess whether Ff phages accumulated in the central nervous system and lungs following intranasal administration can inhibit orthotopic glioblastoma and lung carcinoma progression.


The effect of Ff phages on tumor growth and survival was evaluated in subcutaneous as well as orthotopic models of GL261 glioblastoma and Lewis lung carcinoma using immunocompetent mice.


We demonstrate that Ff phages that do not display any proteins or peptides could inhibit the growth of subcutaneous tumors in mice and that this activity is mediated in part by lipopolysaccharide molecules attached to their virion. Ff phages administered via the intranasal route rapidly accumulated in the brains and lungs of mice and could attenuate progression of orthotopic glioblastoma and lung carcinoma. The anti-tumorigenic activity mediated by the phages was associated with prolonged survival.


This study demonstrates the feasibility of delivering Ff phages non-invasively via the intranasal route to treat brain and lung malignancies. We propose that the anti-tumorigenic activity of Ff phages depends on an effective amount of virion associated lipopolysaccharide which can promote pro-inflammatory activity resulting in tumor suppression.

Clinical trial identification


All authors have declared no conflicts of interest.