1395P - A preliminary study of the effect and mechanism of 1,25 (OH) 2D3 on lung adenocarcinoma cell proliferation and differentiation

Date 30 September 2012
Event ESMO Congress 2012
Session Poster presentation II
Topics Basic Science
Lung and other Thoracic Tumours
Presenter Rong Li
Authors R. Li1, Y. Lou2, L. Xiong2, A. Gu2, B. Han2, Q. Dong3
  • 1Department Of Pulmonary Medicine, 1 Shanghai Chest Hospital affiliated to Shanghai Jiaotong University, 200030 - Shanghai/CN
  • 2Department Of Pulmonary Medicine, Shanghai Chest Hospital affiliated to Shanghai Jiaotong University, 200030 - Shanghai/CN
  • 3Cancer Stem Cells And Tumor Diagnosis, Shanghai Cancer Institute, 200032 - Shanghai/CN

Abstract

Background

OCT4 + bronchioloalveolar stem cell (BASC) is a type of cancer stem cell (CSC)-like cell, and threat to the survival of lung cancer patients. Vitamin D has a certain role in tumor prevention and treatment, but the relationship between vitamin D and lung adenocarcinoma is still unclear.

Objective

To make clear if lung cancer patients occur with vitamin D deficiency in China. Analyze the relationship between vitamin D levels and the pathology. To analyze the role of vitamin D on the differentiation of CSC-like, AT2-like and AT1-like lung adenocarcinoma cells.

Methods

Chemiluminescence detection was used to detect serum 25(OH)D concentrations. The expression of CCSP, SP-C, OCT4, SP-B on CSC-like, AT2-like and AT1-like lung adenocarcinoma cells were detected by immunofluorescence detection. The CCK8 assay and the immunofluorescence detection were used separately to detect proliferation activity and the expression of OCT4 for the OCT4 +BASC.

Results

The mean serum 25(OH)D concentration of 197 cases with lung cancer in China was 10.63 ± 7.04ng/mL; the proportion of vitamin D deficiency was 173/197 in all patients, 103/112 in adenocarcinoma. and 31/39 in squamous cell carcinoma. The vitamin D deficiency ratio was higher in adenocarcinoma than in squamous cell carcinoma, p= 0.043. PGJ2DFOM can induce OCT4 + BASC to be CCSP-SP-C + OCT4- cell, and then 1,25(OH)2D3 can prevent the expression re-recovery of OCT-4 and CCSP. DAPT significantly lowered CCSP expression in OCT4 + BASC, and then 1,25(OH)2D3 can make it become OCT4-SP-C-CCSP-SP-B+ cell, which is a mature AT2-like cell. 1,25(OH)2D3 has no effect on OCT4 + BASC or AT1-like cell differentiation.

Conclusion

Lung cancer patients are generally lacking vitamin D, and lung adenocarcinoma patients are more likely to be lacking vitamin D. 1,25(OH)2D3 can promote lung adenocarcinoma progenitor cell differentiation.

Disclosure

All authors have declared no conflicts of interest.