P-200 - Real time image of mitochondria in living colon cancer tissues using probe based multiphoton microscopy

Date 04 July 2015
Event WorldGI 2015
Session Posters
Topics Basic Science
Colon Cancer
Presenter E.S. Kim
Citation Annals of Oncology (2015) 26 (suppl_4): 1-100. 10.1093/annonc/mdv233
Authors E.S. Kim1, H.S. Choi1, H.J. Chun1, I.K. Yoo1, J.M. Lee1, S.H. Kim1, B. Keum1, Y.T. Jeen2, B.R. Cho3
  • 1Korea University College of Medicine, Seoul/KR
  • 2Department of Internal Medicine, Seoul/KR
  • 3Korea University, Seoul/KR

Abstract

Introduction

Multiphoton endomicroscopy is the recently updated technique for endoscopy and virtual image and optical sectioning. However optimized probe has not been established for multiphoton endomicroscopic image. Therefore we developed novel probe for mitochondria and applied for colon neoplasm tissues. In cancer cell, abnormally increased mitochondrial replication is related mitochondrial dysfunction and Warburg effect.

Methods

We used newly developed multiphoton probe for mitochondria imaging which are made using benzofuran derivative (BFP, maximal multiphoton fluorescence at 570 nm, Figure 1). Fresh mucosal tissues of colonic adenoma and adenocarcinoma were obtained from endoscopic biopsy. Multiphoton probe BFP for mitochondria was stained for tissues and imaging performed using multiphoton microscopy.

Results

BFP shows high enhancement factor upon binding mitochondria, good selectivity, cell permeability, and can readily detect mitochondria in human tissues by multiphoton microscopy. Mitochondria were detected in human colon mucosa tissues. Calculated mitochondria area were increased in adenocarcinoma tissues compared to normal mucosal tissues.

Conclusion

Newly developed multiphoton probe for mitochondria are usable to image human live colon tissues.

Figure: P-200