P-0008 - Effect on the proliferation, invasion and metastasis of gastric cancer cells transfected by metastasis suppressor gene kiss-1 in vivo
|Date||28 June 2014|
|Event||World GI 2014|
|Topics|| Basic Science
|Citation||Annals of Oncology (2014) 25 (suppl_2): ii14-ii104. 10.1093/annonc/mdu165|
Recent studies have found kiss-1 gene to be a new tumor metastasis suppressor gene, associated with a variety of tumor invasion and metastasis. Our previous study found that kiss-1 gene can suppress the proliferation, colony formation, migration and invasion of gastric cancer cells in vitro. This study aimed to further explore the effects of kiss-1 gene on gastric cancer growth and organic metastasis in vivo, as well as investigate related mechanisms.
In this study, the growth of subcutaneous xenografts and lung, liver metastasis of hematogenous disseminated gastric cancer cell in nude mice was used to evaluate the effect on the growth and metastasis of gastric cancer cells transfected by kiss-1 gene in vivo. We detected the levels of Ki67, Sp1 and VEGF protein by immunohistochemical staining and evaluated microvessel density (MVD) by CD31 marker on subcutaneous xenografts. Liver and lung metastases count were used to evaluate the effect on organic metastasis of kiss-1 gene.
Kiss-1 gene significantly suppressed the growth of gastric xenografts and lung, liver metastasis of hematogenous disseminated gastric cancer cell in nude mice, reduced Sp1, VEGF protein expression and xenografts microvessel density in vivo.
Kiss-1 gene significantly suppressed the growth and metastasis of gastric cancer cell in nude mice, mechanisms of which were involved with that kiss-1 gene down-regulated transcription factor Sp1 and inhibited VEGF transcription, thereby suppressed tumor angiogenesis.