32P - Helicobacter pylori CagA expression is closely associated with tumor PD-L1 expression and the better prognosis of gastric cancer patients (32P)

Date 08 December 2017
Event ESMO Immuno-Oncology Congress 2017
Session Lunch & Poster Display session
Topics Cancer Immunology and Immunotherapy
Head and Neck Cancers
Presenter Sung-Hsin Kuo
Citation Annals of Oncology (2017) 28 (suppl_11): xi6-xi29. 10.1093/annonc/mdx711
Authors S. Kuo1, M. Wu2, J. Liou2, C. Shun3, M. Wei1, Y. Zeng1, P. Hsu2, A. Cheng1
  • 1Department Of Oncology, National Taiwan University Hospital, 100 - Taipei/TW
  • 2Department Of Internal Medicine, National Taiwan University Hospital, 100 - Taipei/TW
  • 3Department Of Pathology, National Taiwan University Hospital, 100 - Taipei/TW

Abstract

Background

We recently found that gastric cancer patients with H. pylori (HP) infection had a better overall survival (OS) than those without. In HP-co-cultured lymphoma cells, we found that HP cytotoxin-associated gene A (CagA) up-regulated expression of phospho (p)-SHP-2 and p-ERK, and immune molecules of PD-L1, and FOXP3. In this study, we assessed whether CagA expression is associated with the expression of PD-L1 or FOXP3 in tumor cells, and clinical outcomes of patients with gastric cancer.

Methods

HP strains were cultured from patients with HP-dependent gastric lymphoma. We co-cultured gastric epithelial cells (GECs) with HP strains and further evaluated the expression patterns of CagA, p-SHP-2, p-ERK, PD-L1, and FOXP3 in GECs. The association between CagA expression and expression patterns of PD-L1 and FOXP3 in tumor cells by immunohistochemistry, clinical stage, and OS were further evaluated in 112 patients with stage I to III gastric cancer.

Results

In HP-co-cultured GECs, we revealed that CagA translocated into nucleus and further up-regulated the expression of p-SHP-2, p-ERK, PD-L1, and FOXP3 in GECs. In tumor samples of gastric cancers, we found that CagA was detected in tumor cells of 43 (38.4%) cases. The CagA expression was closely associated with the stage I/II (p = 0.05) and the expression of PD-L1 (p 

Conclusions

CagA can up-regulate the expression of PD-L1 and tumor-infiltrating FOXP3 and thus contribute to the better survival of HP-positive gastric cancer. Further investigation of the association between tumor PD-L1 expression and aggressive behavior of HP CagA-negative gastric cancer is warranted.

Clinical trial identification

Not applicable.

Legal entity responsible for the study

The Ministry of Science and Technology, Taiwan.

Funding

None

Disclosure

All authors have declared no conflicts of interest.