15P - The n-hexane fraction of Myrmecodia pendans suppresses survival and proliferation in colon cancer cells (15P)

Date 18 November 2017
Event ESMO Asia 2017 Congress
Session Poster lunch
Topics Basic Science
Presenter Yasmin Anissa Ruswandi
Citation Annals of Oncology (2017) 28 (suppl_10): x1-x6. 10.1093/annonc/mdx652
Authors Y.A.R. Ruswandi1, S. Hidayat2, F. Huda3, T. Putri4, N. Qomarilla4, D. Kurnia5, M. Satari6, M.H. Bashari7
  • 1Undergraduate Program, Faculty Of Medicine, Universitas Padjadjaran, 40161 - Bandung/ID
  • 2Undergraduate Program, Faculty Of Medicine, Faculty of Medicine, Universitas Padjadjaran, 40161 - Bandung/ID
  • 3Anatomy, Physiology, And Cellular Biology, Faculty of Medicine, Universitas Padjadjaran, 40161 - Bandung/ID
  • 4Laboratory Of Cell Culture And Cytogenetics, Faculty of Medicine, Universitas Padjadjaran, 40161 - Bandung/ID
  • 5Chemistry, Faculty of Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, 45363 - Bandung/ID
  • 6Oral Biology, Faculty of Dentistry, Universitas Padjadjaran, Bandung/ID
  • 7Pharmacology And Therapy, Faculty of Medicine, Universitas Padjadjaran, 40161 - Bandung/ID

Abstract

Background

Colon cancer is a major cause of morbidity and mortality in the world. Some patients develop resistance to chemotherapeutic agents, therefore discovering a novel anti-cancer agent is urgently needed. Myrmecodia pendans, sarang semut (local name), is one of Indonesia’s potential natural resources for cancer therapy that has been studied in some cancer entities. The aims of this study were to determine anti-tumor activities of sarang semut in colon cancer cell lines.

Methods

Anti-tumor activities of sarang semut were evaluated in Caco-2 and HCT-116 cells using MTT assay for cell death and inhibitory concentration (IC50), clonogenic assay for plating efficiency (%), and colony area per cell seeded (mm2) as well as serial trypan blue exclusion assay for doubling time and cell growth curve. Data were considered significantly different if p 

Results

Survival of Caco-2 cells was prominently decreased by the n-hexane fraction of sarang semut than the methanol extract or ethyl acetate fraction. The IC50 of the n-hexane fraction was 24 ppm and 30 ppm in Caco-2 and HCT-116 cells, respectively. Moreover, the n-hexane fraction of sarang semut inhibited colony formation in Caco-2 cells, shown by the difference of plating efficiency (p 

Conclusions

The n-hexane fraction of sarang semut demonstrates potent antitumor activity in colon cancer cells; in particular it inhibits cell survival and proliferation.

Clinical trial identification

Legal entity responsible for the study

Muhammad Hasan Bashari

Funding

Fundamental Research Grant from Universitas Padjadjaran for MHB (no.855/UN6.3.1/PL/2017).

Disclosure

All authors have declared no conflicts of interest.