17P - Endocrine Disrupting chemicals, Bisphenol A alters cardiomyocytes beating rate and cell morphology (17P)

Date 18 November 2017
Event ESMO Asia 2017 Congress
Session Poster lunch
Topics Basic Science
Presenter Siti Hamimah Sheikh Abdul Kadir
Citation Annals of Oncology (2017) 28 (suppl_10): x1-x6. 10.1093/annonc/mdx652
Authors S.H. Sheikh Abdul Kadir1, Z. Rasidi2, N.I. Hanafi1, R. Kamaludin3, S. Ab. Rahim1, R. Siran1, M.H.D. Othman3
  • 1Institute Of Medical Molecular Biotechnology, Faculty Of Medicine, Universiti Teknologi MARA, 47000 - Sungai Buloh/MY
  • 2Centre Of Preclinical Sciences Studies, Faculty Of Dentistry, Universiti Teknologi MARA, 47000 - Sungai Buloh/MY
  • 3Advanced Membrane Technology Research Centre (amtec), Universiti Teknologi Malaysia, 81310 - Johor Bharu/MY

Abstract

Background

Endocrine disrupting chemicals such as Bisphenol A (BPA) has the potential to cause adverse effects to health by disrupting normal human homeostasis. BPA is widely used plasticizer and it is an ideal polymer for epoxy resins and polycarbonates. Therefore, humans are regularly exposed to this chemical through ingestion of water, food and beverages which are contaminated with BPA. Studies have linked BPA exposure in humans with the risk of cardiovascular disease development, yet the direct effects of BPA on cardiomyocytes morphology have not been entirely explored. In here we aimed to investigate the cytotoxic effects of BPA on cells structure of isolated neonatal rat cardiomyocytes culture.

Methods

Cardiomyocytes were isolated from newborn Sprague Dawley rats. Cardiomyocytes were treated with 10-7 to 10-4 M of BPA and subjected for beating rates experiment, cell viability assay and Scanning Electron microscopy. In beating rate experiment, significant reduction in rates (50% -55%, ± 1.5275, p ≤ 0.05) were observed in cardiomyocytes treated with 10-7 to 10-4 M of BPA.

Results

In beating rate experiment, significant reduction in rates (50% -55%, ± 1.5275, p ≤ 0.05) were observed in cardiomyocytes treated with 10−7 to 10−4 M of BPA. Interestingly, cell viability was markedly reduced (54%, ± 0.0026, p ≤ 0.05) in cardiomyocytes treated with 10−7 M of BPA compared to cells in untreated group and others BPA concentration. Cardiomyocytes show altered cell surface homogeneity after BPA exposure. The signs of flattening cardiomyocytes cell surface, reduction of the size, and blurring of the cell borders were observed and evident after exposure to 10−7 to 10−4 M of BPA.

Conclusions

This study provides in vitro evidence of the potential adverse effects of BPA on cardiomyocytes morphology. However, further investigation would be required to understand how BPA is likely to be potentially hazardous to heart and other heart cells.

Clinical trial identification

Legal entity responsible for the study

Universiti Teknologi MARA Animals Ethics Committee

Funding

None

Disclosure

All authors have declared no conflicts of interest.