140PD - The role of leptin and its receptor in oligo-astrocytic neoplasms

Date 17 December 2016
Event ESMO Asia 2016 Congress
Session CNS tumours
Topics Central Nervous System Malignancies
Translational Research
Presenter Ramya Vokuda
Citation Annals of Oncology (2016) 27 (suppl_9): ix42-ix45. 10.1093/annonc/mdw578
Authors R.S. Vokuda1, S. Bh2, V.S. Madhugiri3, S. Kumar2
  • 1Pathology, Jawaharlal Institute of Postgraduate Medical Education and Research, 605006 - Puducherry/IN
  • 2Pathology, Jawaharlal Institute of Postgraduate Medical Education and Research, 605006 - Pondicherry/IN
  • 3Neurosurgery, Jawaharlal Institute of Postgraduate Medical Education and Research, 605006 - Pondicherry/IN

Abstract

Background

Leptin is the first member of the adipocytokines with a molecular weight of 16kDa. It functions in tandem with the leptin receptor, OB-R isoform which contains an intact intracellular domain that has the ability to activate the JAK/STAT pathway. It stimulates growth, migration, invasion of cancer cells in vitro and also potentiates angiogenesis justifying the malignant nature of leptin. Recently, leptin has been explored as an angiogenic entity, thus influencing several signalling systems involved in progression or development of tumors. Despite having advanced therapy regimens for the high grade tumors, they exhibit poor prognosis and low survival rates. Therefore search for novel therapy regimens has resulted in discovery of many potential biomarkers & pharmaceutical targets. In this regard we analysed the potential role of leptin and its receptor in carcinogenesis of malignant gliomas.

Methods

Fresh tissue samples of 60 cases of malignant astrocytoma/Oligodendroglioma were collected after the excision of tumor with informed consent. Real time PCR and immunohistochemical (IHC) analysis were carried out to assess the expression of leptin and its receptor.

Results

The present study demonstrates that the leptin and its receptor is over-expressed in malignant gliomas and could be involved in tumor progression. Of the total 60 cases, 57 cases (95%) and 55 cases (91.6%) showed amplification for leptin & OB-R respectively on real time PCR analysis. On IHC, the expression of these proteins were measured quantitatively and correlated with degree of malignancy which was also statistically significant (p 

Conclusions

The real time PCR analysis for these genes has been done for the first time in Oligo-Astrocytic neoplasms using fresh tissue samples. It represents the first step towards understanding the molecular pathway of leptin and its receptor in these tumors. Leptin may be valued as a pharmaceutical target in malignant gliomas. Anti-leptin compounds could be developed as drugs in mono- or combined therapies for these tumors.

Clinical trial indentification

Legal entity responsible for the study

Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Pondicherry

Funding

Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Pondicherry

Disclosure

All authors have declared no conflicts of interest.