341P - The role and significance of myeloid-derived suppressor cells-like blasts in immune-tolerance of acute myeloid leukemia

Date 18 December 2016
Event ESMO Asia 2016 Congress
Session Poster lunch
Topics Leukaemia
Cancer Immunology and Immunotherapy
Presenter Shin Young Hyun
Citation Annals of Oncology (2016) 27 (suppl_9): ix104-ix111. 10.1093/annonc/mdw586
Authors S.Y. Hyun1, J. Cheong2
  • 1Internal Medicine, Yonsei University Wonju College of Medicine, 26426 - Wonju/KR
  • 2Internal Medicine, Yonsei University College of Medicine Department of Internal Medicine, 03722 - Seoul/KR

Abstract

Background

Myeloid-derived suppressor cells (MDSCs) have an ability to suppress T-cell function and have been known to facilitate tumor growth. We elucidated the role of leukemic subpopulation which resembles MDSC in acute myeloid leukemia.

Methods

CD11b+CD33+HLA-DR- blast (MDSC like blast) were isolated using flow-cytometry from bone marrow mononuclear cells of each patients. CD14, CD15, Arg1 and iNOS expression were checked by flow-cytometry. To confirm the immunosuppressive function, co-culture with normal CD8+ T cell with/without PHA was conducted. A clinical impact was reviewed in 63 patients with retrospective chart review.

Results

MDSCs like blast can be divided into two subtypes, monocytic subgroup expressing CD14 and granulocytic subgroup expressing CD15, and CD14 expression was more frequent than CD15 (67.5% vs. 39.3%). MDSC-like blasts showed higher expression of Arg1 (77.1% vs. 38.5%, P 

Conclusions

CD33+HLA-DR- MDSC-like blasts subgroup existed in myeloid blasts and suppressed T cell immunity. It also showed adverse prognostic effect on survival. MDSC-like blasts might play a certain role in immune-tolerance in leukemia.

Clinical trial indentification

Institutional Review Board of Severance (Clinical Trial Number 4-2010-0669)

Legal entity responsible for the study

Institutional Review Board of Severance Hospital(Clinical Trial Number 4-2010-0669

Funding

This research was supported by Basic Science Research Program through the National Research Foundation of Korea(NRF) funded by the Ministry of Science, ICT & Future Planning(2016-52-0077)

Disclosure

All authors have declared no conflicts of interest.