331P - Synergistic effects of ferula gummosa and radiotherapy to induce cytotoxicity and apoptosis in the hela cell line

Date 18 December 2016
Event ESMO Asia 2016 Congress
Session Poster lunch
Topics Basic Science
Gynaecologic Malignancies
Surgery and/or Radiotherapy of Cancer
Presenter azar Fani-Pakdel
Citation Annals of Oncology (2016) 27 (suppl_9): ix94-ix103. 10.1093/annonc/mdw585
Authors S.H. Forouzmand1, S.H. Mousavi2, V. Vazifedan1, M. Nourbakhsh3, S. Soleymanifard4, J. Chamani1, A. Fani-Pakdel3
  • 1Department Of Biochemistry And Biophysics, Islamic Azad University, 000000000 - Mashhad/IR
  • 2Department Of Pharmacology And Pharmacological, Mashhad University of Medical Sciences, 000000000 - Mashhad/IR
  • 3Cancer Research Center, Mashhad University of Medical Sciences-Omid Hospital Cancer Research Center, 000000000 - Mashhad/IR
  • 4Department Of Medical Physics, Mashhad University of Medical Sciences, 000000000 - Mashhad/IR

Abstract

Background

Cervical cancer is the second most common type of cancer among women worldwide, for which radiotherapy is a standard treatment. Ferula gummosa Boiss (Apiaceae) is an extremely precious medicinal plant which naturally grows throughout the Mediterranean and Central Asia. The present study examined the cytotoxic effects on HeLa cells from the induction of apoptosis and radiation sensitivity by Ferula gummosa.

Methods

HeLa cells were cultured in a RPMI1640 medium, incubated with different concentrations of Ferula gummosa (0-250 µg/ml), and afterwards exposed to 2 Gy γ-rays. The cytotoxicity effect was determined by the MTT assay. Via flow cytometry, apoptotic cells were quantified by apropidium iodide (PI) staining of DNA fragmentation.

Results

Ferula gummosa decreased cell viability in the HeLa cell line in a concentration- and time - dependent manner. Ferula gummosa compared to the control induced a sub-G1 peak in the flow cytometry histogram of treated cells thus indicating that apoptotic cell death is involved in Ferula gummosa-induced toxicity. It was also shown it can sensitize cells to radiation-induced toxicity and apoptosis.

Conclusions

The concurrent use of Ferula gummosa and radiation increases radiation sensitivity and cell death. Therefore, Ferula gummosa can be considered as a potential agent and radiosensitizer in the medical treatment of cancer.

Clinical trial indentification

Legal entity responsible for the study

N/A

Funding

Mashhad University of Medical Sciences

Disclosure

All authors have declared no conflicts of interest.