519P - Lanthanum nanoparticles synthesized from fenugreek seed extract as targeted therapy for osteosarcoma

Date 18 December 2016
Event ESMO Asia 2016 Congress
Session Poster lunch
Topics Bone Sarcomas
Presenter Deblina Dam
Citation Annals of Oncology (2016) 27 (suppl_9): ix163-ix168. 10.1093/annonc/mdw597
Authors D. Dam, K. Banerjee, A. Mukhopadhyay
  • Molecular Biology, Netaji Subhas Chandra Bose Cancer Research Institute, 700016 - Kolkata/IN

Abstract

Background

Osteosarcoma is the most common primary malignant bone tumor accounting for 4% of all childhood cancers worldwide (1). The incidence varies from 4.7% to 11.6% in India. Fenugreek seeds (Trigonella Foenum Graecum) have long been known for their capability to protect tissues from cancer. Aim of our study was to analyze the anticancer potential of Fenugreek seeds in human osteosarcoma cell line (MG-63).

Methods

MTT metabolic activity assay was used. Briefly, MG 63 (1 × 106 cells/ml) were cultured in a T-75 flask and transferred to a 96-well plate (1x104cells/well) and kept for one day at 37°C, then they were exposed to varying concentrations of Fenugreek seed extract.

Results

FTIR spectrum studies were carried out to investigate the possible bioreducing functional groups present. In the FTIR spectra peak was observed in the 3356.14 cm range, confirming the presence of amine functional group. The SEM analysis was used to determine the structure of the reaction products that were formed. SEM image has showed individual Lanthanum particles as well as a number of aggregates. The SEM image showed relatively spherical shape nanoparticle formed with diameter range. Lanthanum nanoparticles were screened for antibacterial activity against drug resistant bacterial strains by well diffusion technique. The highest antibacterial activity was seen for Serratia sp. The anti-oxidant property was checked: Standard absorbance: 0.823; TN absorbance: 0.324 Dose dependent analysis of viability of human osteosarcoma cell line (MG 63) showed 50% lethal toxicity of lanthanum nano particles (solution) of concentration 100 mg/ml.

Conclusions

Lanthanum nanoparticles were successfully synthesized from Fenugreek seed (Trigonella Foenum Graecum) extract. In the present study I found that seeds can be a good source for the synthesis of lanthanum nanoparticles. Based on our result we can conclude that Fenugreek seed can be used as an anti-cancer agent. Plant mediated synthesis of metal nanoparticles is gaining more importance owing to its simplicity, economic viability and rapid rate of synthesis of nanoparticles of attractive and diverse morphologies and elimination of elaborate maintenance of cell cultures and eco-friendliness.

Clinical trial indentification

NA

Legal entity responsible for the study

N/A

Funding

Netaji Subhas Chandra Bose Cancer Research Institute

Disclosure

All authors have declared no conflicts of interest.