16P - Inhibition of invasion and migration by n-3 fatty acids in PC3 cells

Date 17 December 2016
Event ESMO Asia 2016 Congress
Session Poster lunch
Topics Basic Science
Presenter Shoichiro Ohta
Citation Annals of Oncology (2016) 27 (suppl_9): ix1-ix8. 10.1093/annonc/mdw573
Authors S. Ohta1, K. O-Ono1, T. Matsumura2, S. Taniguchi3
  • 1Faculty Of Pharmaceutical Science, Josai University, 3500295 - Sakado/JP
  • 22. institute For Biomedical Sciences, Interdisciplinary Cluster For Cutting Edge Research, Shinshu University, School of Medicine, 390-8621 - Matsumoto/JP
  • 34. department Of Comprehensive Cancer Therapy, Shinshu University, School of Medicine, 390-8621 - Matsumoto/JP

Abstract

Background

Recently, n-3 fatty acids have been shown to decrease the proliferation of cancer cells in colon cancer, breast cancer, hepatitic cancer, and neuroblastoma. Many studies have been carried out in relation to the growth of cancer cells. But few studies have examined invasion and metastasis in prostate cancer. Therefore, in this study, we investigated whether n-3 fatty acids (EPA and DHA) inhibit the proliferation, invasion, and migration of PC3 cells, which is an androgen-independent human prostate cancer cell line that is similar to castration-resistant prostate cancer. Our study was intended to explore a possible method for treating new castration-resistant prostate cancer.

Methods

PC3 cells were cultured, and various concentrations of EPA or DHA were added. Cancer proliferation was confirmed by trypan blue microscopy. Invasion and migration assay were used in the upper chamber in PC3 cells, and serum-free medium and various concentrations of EPA or DHA were placed under plate in serum medium.

Results

The effect of EPA on PC3 cells was dose-dependent; it was possible to obtain significant differences at concentrations of 100 and 200 µg/mL. The effect of DHA on PC3 cells has become the same as for EPA. In the migration assay, EPA showed almost identical results to the control at 25 µg/mL, but migration was reduced at 50 µg/mL. DHA showed the same results as EPA at 25 µg/mL and further reduction was observed at the 50µg/mL concentration. In the invasion assay, EPA did not show any difference to the control at a concentration of 25µg/mL, but suppressed the invasion at 50 µg/mL. DHA resulted in decreased invasion compared with the control at 25 µg/mL, and invasion was significantly reduced at a DHA concentration of 50 µg/mL.

Conclusions

These substances restrained invasion and migration as well as proliferation of PC-3 cells. Their detailed mechanisms are not known, but part of the route has been elucidated.

Clinical trial indentification

This data is not clinical study.

Legal entity responsible for the study

Shoichiro Ohta

Funding

My Grants

Disclosure

All authors have declared no conflicts of interest.