17P - Ethanol extract of Lophatheri Herba exhibits in vitro and in vivo anti-metastatic and anti-angiogenic activities in malignant cancer cells

Date 17 December 2016
Event ESMO Asia 2016 Congress
Session Poster lunch
Topics Basic Science
Presenter Aeyung Kim
Citation Annals of Oncology (2016) 27 (suppl_9): ix1-ix8. 10.1093/annonc/mdw573
Authors A. Kim, M. Im, E. Park, J.Y. Ma
  • Korean Medicine (km) Application Center, Korea Institute of Oriental Medicine (KIOM), 41062 - Daegu/KR

Abstract

Background

Lophatheri Herba (LH), the dried leaf of Lophatherum gracile Brongn, has long been used to reduce thirst and treat fever and inflammation in Chinese medicine. Recent studies have shown that LH has anti-viral, anti-bacterial, anti-cancer, anti-oxidant, diuretic, and hyperglycemic properties. However, the effects of an ethanol extract of L. herba (ELH), at non-cytotoxic doses, on the metastatic and angiogenic abilities of malignant tumor cells have not been reported.

Methods

In the present study, we examined the influence of an ethanol extract of LH (ELH) on the metastatic and angiogenic properties of malignant tumor cells by using in vitro assays, an in vivo pulmonary metastasis model, ex vivo aortic ring assays, and in ovo chick chorioallantoic membrane (CAM) assays. Furthermore, we studied the underlying mechanisms of the anti-metastatic and anti-angiogenic activities of ELH and determined whether ELH suppresses tumor growth by using an in ovo xenograft model and whether it has the potential to control malignant tumors.

Results

We found that ELH significantly suppressed p38, JNK, and NF-κB activation and proteolytic activities under phorbol 12-myristate 13-acetate (PMA) stimulation, thus leading to a decrease in metastatic potential, including migration and invasion. In addition, ELH suppressed tumor-induced angiogenesis, including migration and tube formation in HUVECs and microvessel sprouting from aortic rings via decreasing the pro-angiogenic factors in tumors. Interestingly, in ovo xenografts ELH-treated HT1080 cells did not increase in volume and eventually disappeared, owing to a lack of angiogenesis. Daily oral administration of ELH at 50 and 100 mg/kg markedly inhibited metastatic colonization of B16F10 cells in the lungs of C57BL/6J mice and caused no apparent side effects.

Conclusions

These data collectively indicate that ELH is safe and may be useful for managing the metastasis and growth of malignant cancers.

Clinical trial indentification

none

Legal entity responsible for the study

N/A

Funding

Grant K15280 awarded to Korea Institute of Oriental Medicine (KIOM) from Ministry of Science, ICT and Future Planning (MSIP), Republic of Korea

Disclosure

All authors have declared no conflicts of interest.