9P - Anti-tumor effects mediated by the activation of natural cytotoxicity receptors of natural killer cells

Date 17 December 2016
Event ESMO Asia 2016 Congress
Session Poster lunch
Topics Basic Science
Presenter Ho Young Kim
Citation Annals of Oncology (2016) 27 (suppl_9): ix1-ix8. 10.1093/annonc/mdw573
Authors H.Y. Kim1, K.H. Lee2, J.S. Song3, K.Y. Shim4, J.I. Lee4
  • 1Hematooncology, Internal Medicine, Hallym University Medical Center, 431-070 - Anyang/KR
  • 2Microbiology, Yonsei University Wonju College of Medicine, 220-701 - Wonju/KR
  • 3Pathology, Catholic Kwandong University International St. Mary’s Hospital, 22711 - Incheon Metropolitan City/KR
  • 4Hematooncology, Internal Medicine, Yonsei University Wonju Severance Christian Hospital, 220-701 - Wonju/KR

Abstract

Background

The natural killer cell (NK cell) is very important for the removal of cancer cells in the stage of tumor growth and metastasis as well as in the early stage of the biologic immune system. The tumor killing system of NK cell can be induced by antibody-dependent cell-mediated cytotoxicity (ADCC) and natural cytotoxicity receptors (NCR)-mediated natural killing mechanism.

Methods

In this study, the anti-cancer effect of NK cells through the promotion of cytotoxicity was demonstrated in the experiment of human hepatocellular carcinoma cell lines, HepG2 and Hep3B using GÖ6983, a molecular substance to induce NCR expression of NK cells. A lung metastasis mouse model was made in C57BL/6 mouse aged 8 weeks with a mouse malignant melanoma cell line, B16BL6.

Results

The results are as follows. 1) The expression of NCR1, 2, and 3 with the treatment of GÖ6983 increased 4.11, 57.34 and 9.89 times, respectively compared with those without treatment. 2) The cytotoxicity of NK cells with the treatment of GÖ6983 showed destruction of 22.3% in Hep3B and 21.6% in HepG2 compared with 10% in control cases. 3) In lung metastasis mouse model, ① Activated NK cells revealed 43.2% reduction of metastastic lung nodules in cases treated with the dose of 10μM and 51.2% reduction in 50 μM compared with control cases (p 

Conclusions

In conclusion, treatment of GÖ6983 to the NK cell showed activation of NCR1, 2, and 3 that might mean indirect activation of NK cells. Moreover, induction of activated NK cells in the pretreatment group resulted in significant anti-metastatic effect and improved survival.

Clinical trial indentification


Legal entity responsible for the study

HY Kim

Funding

N/A

Disclosure

All authors have declared no conflicts of interest.