9P - Anti-tumor effects mediated by the activation of natural cytotoxicity receptors of natural killer cells
|Date||17 December 2016|
|Event||ESMO Asia 2016 Congress|
|Topics|| Basic Science
|Presenter||Ho Young Kim|
|Citation||Annals of Oncology (2016) 27 (suppl_9): ix1-ix8. 10.1093/annonc/mdw573|
H.Y. Kim1, K.H. Lee2, J.S. Song3, K.Y. Shim4, J.I. Lee4
The natural killer cell (NK cell) is very important for the removal of cancer cells in the stage of tumor growth and metastasis as well as in the early stage of the biologic immune system. The tumor killing system of NK cell can be induced by antibody-dependent cell-mediated cytotoxicity (ADCC) and natural cytotoxicity receptors (NCR)-mediated natural killing mechanism.
In this study, the anti-cancer effect of NK cells through the promotion of cytotoxicity was demonstrated in the experiment of human hepatocellular carcinoma cell lines, HepG2 and Hep3B using GÖ6983, a molecular substance to induce NCR expression of NK cells. A lung metastasis mouse model was made in C57BL/6 mouse aged 8 weeks with a mouse malignant melanoma cell line, B16BL6.
The results are as follows. 1) The expression of NCR1, 2, and 3 with the treatment of GÖ6983 increased 4.11, 57.34 and 9.89 times, respectively compared with those without treatment. 2) The cytotoxicity of NK cells with the treatment of GÖ6983 showed destruction of 22.3% in Hep3B and 21.6% in HepG2 compared with 10% in control cases. 3) In lung metastasis mouse model, ① Activated NK cells revealed 43.2% reduction of metastastic lung nodules in cases treated with the dose of 10μM and 51.2% reduction in 50 μM compared with control cases (p
In conclusion, treatment of GÖ6983 to the NK cell showed activation of NCR1, 2, and 3 that might mean indirect activation of NK cells. Moreover, induction of activated NK cells in the pretreatment group resulted in significant anti-metastatic effect and improved survival.
Clinical trial indentification
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All authors have declared no conflicts of interest.