494P - Chemopreventive efficacy of luteolin, a dietary flavone against benzo(a)pyrene induced lung carcinogenesis

Date 19 December 2015
Event ESMO Asia 2015 Congress
Session Poster presentation 1
Topics Cancer Aetiology, Epidemiology, Prevention
Lung and other Thoracic Tumours
Presenter Eshvendar Kasala
Citation Annals of Oncology (2015) 26 (suppl_9): 148-152. 10.1093/annonc/mdv533
Authors E.R. Kasala1, L.N. Bodduluru1, C.C. Barua2, R. Gogoi1, B.K. Bezbarua1, M. Lahkar1
  • 1Pharmacology And Toxicology, NIPER Guwahati, 781032 - Guwahati/IN
  • 2Pharmacology And Toxicology, CVSc, AAU, 781022 - Guwahati/IN

Abstract

Aim/Background

Chemoprevention is regarded as one of the most promising and realistic approaches in the prevention of lung cancer. Several bioactive compounds present in diet such as fruits and vegetables have their beneficial effect on lung cancer. Luteolin is one such naturally occurring dietary phytochemical possesses anti-oxidative, anti-inflammatory and anti-proliferative activity in a wide variety of cancer. The objective of present study is to divulge the chemopreventive role of luteolin against benzo(a)pyrene [B(a)P] induced lung carcinogenesis in Swiss albino mice.

Methods

B(a)P was administered orally (50 mg/kg body weight) twice a week for four weeks to induce lung cancer in mice. Animals were treated with luteolin (15mg/kg body weight, orally) thrice a week for a total duration of 16 weeks. The body weight, lung weight, tumour incidence, lipid peroxidation, activities of tumormarker enzymes (adenosine deaminase, aryl hydrocarbon hydroxylase, ɣ-glutamyl transpeptidase, 5′-nucleotidase and lactate dehydrogenase), antioxidant enzymes (superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase, glutathione reductase, reduced glutathione) and histopathological analysis of lung tissue were carried out. Western blotting analysis of CYP1A1, NF-κB and Nrf2 were also carried out.

Results

Administration of B(a)P to mice resulted in increased lipid peroxides (LPO) with concomitant decrease in the levels of both enzymatic antioxidants such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR), and non-enzymatic antioxidants such as reduced glutathione (GSH), vitamin E and vitamin C. Luteolin supplementation (15 mg/kg body weight) significantly attenuated these alterations thereby showing potent anticancer effect in lung cancer. Further, the anticancer effect of luteolin was confirmed by histopathological and immunoblotting analysis of CYP1A1, NF-κB and Nrf2, where luteolin supplementation modulated the expression of these proteins and maintained cellular homeostasis.

Conclusions

Overall, these findings confirm the chemopreventive potential of luteolin against B(a)P induced lung cancer in Swiss albino mice.

Clinical trial identification

Disclosure

All authors have declared no conflicts of interest.