136P - Association between IL-27 -964A > G gene polymorphism and IL-27p28 levels in Chinese patients with papillary thyroid cancer

Date 19 December 2015
Event ESMO Asia 2015 Congress
Session Poster presentation 1
Topics Thyroid Cancer
Translational Research
Presenter Shulong Zhang
Citation Annals of Oncology (2015) 26 (suppl_9): 40-41. 10.1093/annonc/mdv522
Authors S. Zhang1, Y. Wang2, J. Jia3, Q. Zhang4, Z. Ji5
  • 1Department Of General Surgery, Zhongda Hospital Southeast University, 210009 - Nanjing/CN
  • 2Department Of Interventional Radiology & Vascular Surgery, Zhongda Hospital Southeast University, Nanjing/CN
  • 3Department Of Surgical Oncology, The First Affiliated Hospital of Bengbu Medical College, Bengbu/CN
  • 4Department Of Clinical Science, The First Affiliated Hospital of Bengbu Medical College, Bengbu/CN
  • 5Department Of General Surgery, Zhongda Hospital Southeast University, Nanjing/CN

Abstract

Aim/Background

In this study, we aim to investigate the association between a potentially functional polymorphism (rs153109, -964A > G) at the promoter of IL-27 and the risk of papillary thyroid cancer (PTC) in a Chinese population.

Methods

Genotype of IL-27 -964A > G was determined using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. Serum IL-27p28 levels were determined using enzyme-linked immunosorbent assay (ELISA).

Results

No significant difference was noticed in the IL-27 −964A > G distribution between PTC patients and healthy controls in overall analysis. However, stratified analysis showed that patients carrying the GG genotype or G allele had significantly decreased risks for developing lymph node metastasis compared with patients carrying the AA genotype or A allele (GG vs. AA: OR = 0.42, 95% CI, 0.24-0.73; G vs. A: OR = 0.75, 95% CI, 0.59-0.94). The levels of serum IL-27p28 were significantly decreased in PTC patients compared with those in controls (P < 0.05). Furthermore, ELISA results demonstrated that the GG genotype resulted in up-regulation of IL-27p28 expression compared with those carrying AA genotype or the AG genotype among healthy controls (P < 0.05).

Conclusions

In conclusion, our results suggest that IL-27 -964A > G polymorphism may be associated with lymph node metastasis of PTC, and IL-27p28 may be used as an attractive target for PTC immunotherapy.

Clinical trial identification

Disclosure

All authors have declared no conflicts of interest.