1724P - Some mechanisms of increasing malignancy of B16/F10 melanoma in female mice with chronic pain

Date 11 September 2017
Event ESMO 2017 Congress
Session Poster display session
Topics Basic Science
Melanoma and other Skin Tumours
Presenter Inga Kotieva
Citation Annals of Oncology (2017) 28 (suppl_5): v595-v604. 10.1093/annonc/mdx391
Authors I.M. Kotieva1, O.I. Kit2, E.M. Frantsiyants1, I.V. Kaplieva1, L.K. Trepitaki1, V.A. Bandovkina1, N.D. Cheryarina1, Y.A. Pogorelova1, L.Y. Vladimirova3
  • 1Laboratory Of Study Of Malignant Tumor Pathogenesis, Rostov Research Institute of Oncology, 344037 - Rostov-on-Don/RU
  • 2Department Of Abdominal Oncology, Rostov Research Institute of Oncology, 344037 - Rostov-on-Don/RU
  • 3Drug Therapy Department, Rostov Research Institute of Oncology, 344037 - Rostov-on-Don/RU

Abstract

Background

The impact of chronic pain (CP) on the growth and development of tumors is poorly studied. However, one of the main goals of cancer therapy is the relief of chronic pain, a complex symptom based on combined pathophysiological mechanisms. Our aim was to study the influence of CP on melanoma growth in female mice and to determine levels of the VEGF family members in the tumor (T), its perifocal zone (PZ) and in the skin.

Methods

The study included 64 female C57BL/6 mice; B16/F10 melanoma was transplanted under the skin on the back of animals in the main group 2 weeks after sciatic nerve ligation. Mice with melanoma without CP were used as the controls. Levels of VEGFs: A, C, R1 and R3 were determined by ELISA (BenderMedSystem, Austria).

Results

The life span of mice with melanoma and CP was 1.5 times shorter than the control group. Melanoma in mice with CP was more aggressive, and metastases occurred after 1 week vs. 4 weeks in the controls. The rate of metastasis was higher (100% vs. 60% in the controls); melanoma with CP spread to multiple organs and caused unusual metastases to the heart and uterus. The rapid and specific development of B16/F10 melanoma in mice with CP was accompanied by increased levels of VEGF-A, -C and -R1 in T, PZ and the skin, with their maximal accumulation in T in week 1. The VEGF-A level continued to increase in T and PZ (PZ

Conclusions

CP shortened the life span of female mice with melanoma and enhanced the aggressiveness of B16/F10 melanoma metastases. The activation of angiogenesis in T and PZ can be considered as one of the mechanisms of the neoplastic progression.

Clinical trial identification

Legal entity responsible for the study

Rostov Research Institute of Oncology

Funding

None

Disclosure

All authors have declared no conflicts of interest.