18P - Potential miRNAs involved in molecular pathways mediating the anticancer effects of Short Term Starvation in Breast Cancer cells treated with Doxor...

Date 11 September 2017
Event ESMO 2017 Congress
Session Poster display session
Topics Basic Science
Breast Cancer
Presenter Daniele Fanale
Citation Annals of Oncology (2017) 28 (suppl_5): v1-v21. 10.1093/annonc/mdx361
Authors D. Fanale, L. Incorvaia, R. Maragliano, N. Barraco, A. Listì, A. Galvano, S. Rizzo, V. Calò, V. Bazan, A. Russo
  • Department Of Surgical, Oncological And Oral Sciences, Section Of Medical Oncology, University of Palermo, 90127 - Palermo/IT

Abstract

Background

In recent years, increasing evidences showed that several types of dietary approaches restricting food intake, including Short Term Starvation (STS), may exert a protective role against aging and other age-related pathologies as well as cancer. Interestingly, the dietary restriction showed significant anticancer effects able to prevent cancer onset, slow its progression and improve therapy response. Since recent studies showed that miRNAs may modulate sensibility/resistance to antiblastic therapy, the aim of our study was to investigate the STS-induced molecular changes in breast cancer cells treated with doxorubicin, focusing our attention on miRNA expression profile.

Methods

Vitality assays were used to assess the effects of STS on cell proliferation. Using a TaqMan Low Density Array A human microRNA microarray analysis, the expression profile of 377 miRNAs was analyzed in healthy and malignant breast cells, MCF10A and MDA-MB-231 respectively, treated for 24h with 1µM doxorubicin under STS conditions for 48h. In addition, the expression of mRNAs and miRNAs specifically induced by STS was analyzed in MCF-7, MDA-MB-231 and SkBr3 cells using Real-time PCR analyses.

Results

In vitro cell vitality assays showed that STS, in association with doxorubicin treatment, significantly reduces breast cancer cell proliferation and viability, whereas it appears to protect healthy breast cells from chemotherapeutic treatment. Microarray analysis showed that a subset of miRNAs involved in molecular pathways related to drug sensitivity/resistance was found to be differentially expressed in breast cancer cells following the doxorubicin treatment and STS. Finally, expression analysis of hypothetical miRNA gene targets involved in therapy response have confirmed the coherence of our results.

Conclusions

This work establishes, for the first time, an interesting link between anticancer effects of STS and miRNA expression changes in doxorubicin-treated breast cancer cells, suggesting the potential involvement of some miRNAs in molecular pathways mediating the effects of STS in breast cancer.

Clinical trial identification

Not applicable

Legal entity responsible for the study

Department of Surgical, Oncological and Oral Sciences, Section of Medical Oncology, University of Palermo

Funding

None

Disclosure

All authors have declared no conflicts of interest.