1282P - Characterization of cancer stem cell and immune microenvironment interactions in non-small cell lung cancer (NSCLC).

Date 09 September 2017
Event ESMO 2017 Congress
Session Poster display session
Topics Cancer in Adolescents
Non-Small-Cell Lung Cancer, Early Stage
Lung and other Thoracic Tumours
Translational Research
Presenter Susana Torres
Citation Annals of Oncology (2017) 28 (suppl_5): v453-v456. 10.1093/annonc/mdx381
Authors S. Torres1, R. Sirera2, C. Aguilar1, S. Calabuig Fariñas3, M. Mosqueda1, A. Herreros-Pomares1, E. Escorihuela4, E. García del Olmo5, E. Jantus-Lewintre6, C. Camps Herrero7
  • 1Laboratorio De Oncología Molecular, Fundación para la Investigación, Hospital General Universitario de Valencia, 46020 - Valencia/ES
  • 2Departamento De Biotecnología, Universitat Politècnica de València-CIBERONC, 46022 - Valencia/ES
  • 3Laboratorio De Oncología Molecular, Fundación Para La Investigación, Hospital General Universitario De Valencia-ciberonc;, Departamento de Patología, Universitat de València., 46020 - Valencia/ES
  • 4Laboratorio De Oncología Molecular, Fundación para la Investigación, Hospital General Universitario de Valencia-CIBERONC, 46020 - Valencia/ES
  • 5Cirugía Torácica, Hospital General Universitario de Valencia, 46020 - Valencia/ES
  • 6Laboratorio De Oncología Molecular, Fundación Para La Investigación, Hospital General Universitario De Valencia-ciberonc, Departamento de Biotecnología, Universidad Politécnica de Valencia, 46020 - Valencia/ES
  • 7Laboratorio De Oncología Molecular, Fundación Para La Investigación, Hospital General Universitario De Valencia-ciberonc, Servicio de Oncología Médica, Hospital General Universitario de Valencia; Departamento de Medicina, Universidad de Valencia, 46018 - Valencia/ES

Abstract

Background

Lung cancer stem cells (CSCs) are a small subpopulation of cells with self-renewal, tumorigenic properties and the ability to grow forming tumourspheres in non-adherent conditions. CSCs in non-small cell lung cancer (NSCLC) are targets poorly recognized by the immune surveillance system given that they favour an immunosuppressive microenvironment. The aim of this work was to compare the release of cytokine between monolayer cells and tumourspheres.

Methods

The study was performed on medium supernatant of cells from two NSCLC tumour patients (patient 1 and patient 2) samples and ten cell lines (A549, H1650, H460, H23, H358, H2228, HCC827, PC9, H1993, and SW900) grown in monolayer and tumourspheres at 3 different densities (104, 5·105 and 105 cells/ml). We analysed four soluble factors with immunosuppressive (IL-4, IL-10), and immunoregulatory (IL-6, IL-8) capacity through sensitivity bead-based multiplex assay using the Millipore kit and the Luminex 100/200.

Results

All human tumour cell lines and primary cells secreted detectable levels of IL-6 and IL-8. In contrast, IL-10 and IL-4 levels were below detectable range (1750.122177.4>2273.3H35810.927.81774.8>2273.3H199334.42.2658.2479.3PC9144.45.4597.2321.9SW900121.75.41006225.2H460359.8227.81298.82201.3H233.72.8407.81290.8H1650995.9352.230.250.3Patient 117.40.6>2273.330.0Patient 2>1750.12>1750.12>2273.3>2273.3

Conclusions

Our preliminary results suggest that cells grown in adherence show increased levels of IL-6 compared to lung-tumourspheres. The next step is the expansion of the cohort to obtain significant results.

Supported by grants from FEDER and PI12-02838 and PI15-00753 from ISCIII.

Clinical trial identification

Legal entity responsible for the study

Fundación para la Investigación del Hospital General Universitario de Valencia

Funding

Supported by grants from FEDER and PI12-02838 and PI15-00753 from ISCIII.

Disclosure

All authors have declared no conflicts of interest.