122P - Cell-free (cf)DNA analysis identifies PIK3CA/AKT1 mutations associated with greater PFS improvement from the addition of ipatasertib (IPAT) to pacl...

Date 11 September 2017
Event ESMO 2017 Congress
Session Poster display session
Topics Cancer in Adolescents
Biomarkers
Breast Cancer
Translational Research
Presenter Matthew Wongchenko
Citation Annals of Oncology (2017) 28 (suppl_5): v22-v42. 10.1093/annonc/mdx363
Authors M. Wongchenko1, R. Dent2, S. Kim3, C. Saura4, M. Oliveira4, J. Baselga5, A.V. Kapp6, W.Y. Chan7, S.M. Singel7, D.J. Maslyar7, S. Gendreau8
  • 1Oncology Biomarker Development, Genentech Inc., 9408097124 - South San Francisco/US
  • 2Division Of Medical Oncology, National Cancer Centre, Singapore/SG
  • 3Department Of Oncology, Asan Medical Center, University of Ulsan College of Medicine, Seoul/KR
  • 4Medical Oncology Department, Vall d’Hebron University Hospital, Vall d’Hebron Institute of Oncology, Barcelona/ES
  • 5Human Oncology And Pathogenesis Program And The Department Of Medicine, Memorial Sloan Kettering Cancer Center, New York/US
  • 6Product Development Biostatistics, Genentech Inc., South San Francisco/US
  • 7Product Development Oncology, Genentech Inc., South San Francisco/US
  • 8Oncology Biomarker Development, Genentech Inc., South San Francisco/US

Abstract

Background

The oral Akt inhibitor IPAT is being evaluated in cancers with a high prevalence of PI3K/Akt pathway activation. In the placebo-controlled randomised phase II LOTUS trial (NCT02162719), adding IPAT to P as first-line therapy for metastatic TNBC improved PFS in unselected patients (stratified hazard ratio [HR] 0.60 [90% CI 0.40–0.91]), with a more pronounced effect in patients with PIK3CA/AKT1/PTEN-altered tumours [Dent, ASCO 2017]. cfDNA sequencing was performed to assess the utility of blood samples for detecting tumour mutations in TNBC.

Methods

Pre-treatment plasma and tumour samples were evaluated for genetic alterations using Foundation Medicine’s FoundationACT® and FoundationOne® next-generation sequencing assays, respectively. Samples from 88 patients were evaluable by cfDNA, 72 of whom also had evaluable tumour samples (52 primary tumour). To test the prognostic effect of cfDNA mutational burden, patients were classified as having high or low variant allele fraction (VAF) in cfDNA using the median as a cutoff.

Results

In 81 patients (92%), at least one mutation was detectable by cfDNA sequencing. Concordance with tissue sequencing was 75%. High VAF was associated with shorter PFS than low VAF in both the IPAT + P arm (HR 2.39 [90% CI 1.19–4.70]) and the placebo + P arm (HR 2.68 [90% CI 1.46–5.11]). High VAF was also associated with the presence of > 1 metastatic site but not tumour volume per RECIST v1.1. In 22 patients (25%), an activating PIK3CA (n = 16) or AKT1 (n = 6) mutation was detected in cfDNA; concordance with tissue sequencing was 100%. PFS improvement with the addition of IPAT to P was more pronounced in patients with detectable PIK3CA/AKT1 mutations (HR 0.15 [90% CI 0.03–0.50]) than in those without a detectable mutation (HR 0.82 [90% CI 0.50–1.31]).

Conclusions

These results highlight the potential role of cfDNA in evaluating patient prognosis as well as identifying genetic markers associated with improved treatment outcomes. Furthermore, they support the occurrence of PIK3CA and AKT1 mutations as early genetic events present in primary tissue samples that are maintained during metastasis.

Clinical trial identification

NCT02162719

Legal entity responsible for the study

F Hoffmann-La Roche Ltd.

Funding

F Hoffmann-La Roche Ltd

Disclosure

M. Wongchenko: Employee of Genentech, Inc. and holds shares in Roche and Ariad Pharmaceuticals. R. Dent: Honoraria for consultancy/advisory boards/speaker engagements from Pfizer, Roche, Eisai, Merck, and AstraZeneca. S-B. Kim: Research funding from Novartis, Sanofi-Aventis, Kyowa-Kirin Inc, and Dongkook Pharma Co., Ltd. C. Saura: Honoraria for consulting/advisory roles from Puma Biotechnology, Pfizer, and Roche and research funding (to her institution) from Genentech and AstraZeneca. M. Oliveira: Honoraria for consulting/advisory roles from Puma Biotechnology and Genentech/Roche and research funding (to the institution) from Genentech and AstraZeneca. J. Baselga: Compensation for a leadership role from Infinity Pharmaceuticals, stock or ownership interest in PMV Pharma, Juno Therapeutics, Infinity Pharmaceuticals, and GRAIL, and honoraria for consulting/advisory role for Eli Lilly, Novartis, and GRAIL. A.V. Kapp, W.Y. Chan, S.M. Singel, D.J. Maslyar, S. Gendreau: Employee of Genentech, Inc. and holds stock in Roche.