1505P - Analysis of expression of immunomodulation factors in alveolar soft-part sarcoma: a retrospective study from the Spanish Group for Research on Sarc...

Date 11 September 2017
Event ESMO 2017 Congress
Session Poster display session
Topics Soft Tissue Sarcomas
Sarcoma
Presenter Nadia Hindi
Citation Annals of Oncology (2017) 28 (suppl_5): v521-v538. 10.1093/annonc/mdx387
Authors N. Hindi1, A. Fernandez-Serra2, J. Martinez-Trufero3, A. Carranza-Carranza4, A. Lopez-Pousa5, J. Lavernia6, J.L. Arranz7, R. López Alegret8, J. Martinez-Garcia9, C. Valverde Morales10, J.M. Cano11, M.A. Vaz12, D.S. Moura13, S. Bagué14, R. Gonzalez-Campora15, J.A. Lopez-Guerrero2, J. Martin-Broto1
  • 1Oncología Medica, Hospital Universitario Virgen del Rocio/Instituto de Biomedicina de Sevilla, 41013 - Sevilla/ES
  • 2Laboratoy Of Molecular Biology, Fundación Instituto Valenciano de Oncología, 46009 - Valencia/ES
  • 3Medical Oncology, Hospital Universitario Miguel Servet, 50009 - Zaragoza/ES
  • 4Pathology, Hospital Universitario Virgen del Rocio, Sevilla/ES
  • 5Medical Oncology, Hospital San Pau, Barcelona/ES
  • 6Medical Oncology, Fundacion Instituto Valenciano de Oncologia, Valencia/ES
  • 7Medical Oncology, Fundación Jimenez Diaz, Madrid/ES
  • 8Pathology, Hospital Universitario Miguel Servet, 50009 - Zaragoza/ES
  • 9Medical Oncology, Hospital Universitario Virgen de la Arrixaca, Murcia/ES
  • 10Medical Oncology, Vall d'Hebron University Hospital, 08035 - Barcelona/ES
  • 11Medical Oncology, Hospital General de Ciudad Real, Ciudad Real/ES
  • 12Medical Oncology, Hospital Universitario Ramón y Cajal, Madrid/ES
  • 13Terapias Avanzadas Y Biomarcadores En Oncologia, Instituto de Biomedicina de Sevilla, 41013 - Sevilla/ES
  • 14Pathology, Hospital San Pau, Barcelona/ES
  • 15Pathology, Hospital Universitario Virgen de Macarena, Sevilla/ES

Abstract

Background

Alveolar soft-part sarcoma (ASPS) is an exceedingly rare entity. We previously reported on a series of 12 ASPS the expression of PD-1/PD-L1 and lymphocytic infiltrates. PD-L1 staining was positive in 50% of the cases, suggesting a role for immunomodulatory interventions. We now enlarge the series and explore the expression of immune-related genes with the HTG EdgeSeq System and analyze their possible prognostic role.

Methods

Patients (pts) diagnosed with ASPS from Dec 1994 to Jul 2016 were reviewed. Clinical characteristics and outcome were collected. Immunohistochemical expression was tested on archived Formalin-Fixed Paraffin-Embedded (FFPE) blocks using PD-L1 (ab205921; Abcam), CD8 (ab4055; Abcam) antibodies. An ImmunOncology (IO) panel of 549 mRNAs was evaluated with the HTG EdgeSeq System from one 5µm FFPE section. This novel technology consists in a tissue digestion followed by a pre-hybridization with specific probes using quantitative nuclease protection assay (qNPA) and quantified by a standard RNA-seq protocol in a NGS sequencer.

Results

We identified 16 ASPS pts: median age 23y (6-62), M/F=5/11. Stage localized/metastatic in 11/5. With a median FU of 91 mos (4-151), 6/11 pts (54%) relapsed, with a median RFS of 19 mos (95% CI 1-75) and 2 pts died. PD-L1 was pos in tumor in 10/16 (62%) pts. HTG showed differential expression of immune-related genes according to stage (localized vs metastatic) in MNDA (log2 fold change: -3.01, p 

Conclusions

PD-L1 is expressed in more than a half of ASPS of our series. Differential expression in immune-associated genes suggest an immune related gene profile on this entity with clinical and pathological implications. Further exploration of immune-modulation in ASPS is ongoing.

Clinical trial identification

Legal entity responsible for the study

Spanish Group for Research on Sarcoma

Funding

Spanish Group for Research on Sarcoma (GEIS).

Disclosure

All authors have declared no conflicts of interest.