1025P - Plasmatic Epstein-Barr virus micro-RNA -bart-17 in nasopharyngeal carcinomas patients: high potential as a tumor biomarker associated to EBV DNA con...

Date 01 October 2012
Event ESMO Congress 2012
Session Poster presentation III
Topics Head and Neck Cancers
Translational Research
Presenter Francois Regis Ferrand
Authors F.R. Ferrand1, C. Gourzones2, B. Verillaud2, E. Saada3, P. Lang4, V. Schneider5, C. Amiel5, J. Guigay6, P. Busson2
  • 1Oncologie, Hopital du Val de Grace, 75230 - Paris CEDEX 5/FR
  • 2Umr8126, institut Gustave Roussy, 94805 - villejuif/FR
  • 3Head And Neck Departement, institut Gustave Roussy, villejuif/FR
  • 4Medical Oncology, CHU Pitié-Salpetrière, paris/FR
  • 5Maladies Infectieuses, CHU Tenon, paris/FR
  • 6institut Gustave Roussy, villejuif/FR

Abstract

Background

Because latent Epstein Barr (EBV)-infection is a specific characteristic of malignant Nasopharyngeal carcinoma (NPC) cells, various molecules of viral origin are obvious candidate biomarkers. EBV microRNAs are of particular interest because at least some of these (such as miR-BARTs) deregulate the expression of key cellular and viral genes.

Patients and methods

We performed RT- real-time quantitative PCR targeted on two cellular miRNAs (has miR16 and 146) and two viral miRNA (ebv-miR BART7 and 17-5p) after RNA extraction on plasma samples from 27 advanced NPC patients,10 patients with advanced head and neck Squamous cell carcinoma and 3 EBV healthy carriers.

Results

We showed presence of cellular miRNAs in all the samples, whereas viral miRNAs were mostly found in NPC patients plasma samples, with significant differences for ebv-miR-BART-17 (p = 9,69910−6 Wilcoxon test) but not for miR-BART7 and cellular miRNAs. A detection threshold of 0,3 ebv-miR-BART17 copy/plasma µL was specific for NPC diagnosis (ROC curve analysis (AUC= 0,919) with a sensitivity of 0.9259 and specificity of 0,973) There was no significant relationship between plasma concentrations of either ebv-DNA copy number or viral and cellular miRNAs and studied clinicopahtological factors.

Conclusion

miR-BART-17 concentrations seem specific of nasopharyngeal carcinomas and could bring additional information to viral DNA concentrations especially when DNA is undetectable. Further investigations are needed especially in the context of new generation immunoadpative treatment.

Disclosure

All authors have declared no conflicts of interest.