742P - Investigation of a functional polymorphism in the epidermal growth factor gene for pathogenesis and prognosis of hepatocellular carcinoma in Japanes...
|Date||30 September 2012|
|Event||ESMO Congress 2012|
|Session||Poster presentation II|
|Topics|| Hepatobiliary Cancers
M. Suenaga1, S. Yamada2, T. Fujii3, B.C. Fuchs4, N. Okumura2, G. Nakayama5, S. Takeda2, K.K. Tanabe4, Y. Kodera6
A single nucleotide polymorphism (SNP) in the epidermal growth factor (EGF) has been investigated as a predictor of hepatocellular carcinoma (HCC). Previous reports demonstrated that an A to G transition at position 61 in the 5′ untranslated region of the EGF gene was identified, and that G/G genotype was associated with an increased risk of developing HCC compared with the A/G and A/A genotype. It has been reported that there are differences in the distribution of polymorphisms by race, there is no report in the Japanese population.Objectives
To examine the relationship between EGF gene polymorphism genotype and the development and prognosis of HCC in the Japanese population.Methods
Restriction fragment-length polymorphism was used to determine the EGF gene polymorphism genotype in two hundred and thirteen patients with HCC, who underwent resection in our department between 1996 and 2010. The correlation between these values and clinicopathological factors and prognosis were statistically analyzed. 2) The level of EGF mRNA expression in cancerous tissues was measured by quantitative reverse transcription-PCR. 3) EGF SNP was also analyzed in one hundred and forty patients with hepatitis and liver cirrhosis.Results
1) A/A, A/G, and G/G genotype were 5.2%, 43.7%, and 51.2% in the Japanese patients with HCC. A/A patients had better overall and disease-free prognosis than other genotypes, although no significant differences were found. 2) EGF mRNA expression in G/G patients was significantly higher than that in A/A patients (P = 0.04). 3) A/A, A/G, and G/G genotype were 7.1%, 34.3%, and 58.6% in patients without HCC. Analysis of the distribution of allelic frequencies revealed that odds ratio of developing HCC was 1.76 (95% CI 0.69-4.47, P = 0.23) in A/G patients, and 1.21 (95% CI 0.48-3.00, P = 0.68) in G/G patients, compared with A/A patients.Conclusions
EGF 61*G allele was associated with greater transcript stability and EGF expression in the Japanese population. The EGF gene polymorphism genotype was associated with risk for development of HCC.Disclosure
All authors have declared no conflicts of interest.